general purpose of this lecture is to present on Real Time PCR and It’s Functions In Diagnosis. Here briefly present on Introduction to PCR, Advantages & disadvantages, Real Time PCR, Detection of products, Function in medicine. A real time PCR (polymerase chain reaction) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR, i.e. in real-time, and not at its end, as in conventional PCR.
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